河南农业科学Issue(1)：79-83,5.
美洲南瓜（Cucurbit pepo）WMV 2抗性遗传及分子标记研究
Studies on Inheritance of Resistance to WMV-2 and M olecular M arkers of Pumpkin (Cucurbit pepo)
摘要
Abstract
With 157 pumpkin (Cucurbita pepo) F2 plants as material ,which were generated by hybridi-zing disease-resistant inbred line‘0504’with susceptible inbred line‘0223’ ,the genetic regularity and mo-lecular markers of pumpkin resistance to watermelon mosaic virus 2 (WMV-2)were studied .The pa-rents ,F1 and F2 plants were inoculated with WMV-2 at seedling stage for resistance identification ,and the results showed that the pumpkin resistance to WMV-2 was controlled by a single recessive gene .Bulked separation analysis (BSA) and simple sequence repeats (SSR) technology were utilized jointly and two SSR markers linked to WMV-2 resistance genes ,CMTm 157 and CMTm 158 ,were filtered out .The ge-netic distance of the two SSR markers was 10 .9 cM and 6 .2 cM ,respectively ,which can be used for as-sisted selection in pumpkin anti-WMV-2 breeding .关键词
美洲南瓜/西瓜花叶病毒/抗性遗传规律/SSR标记Key words
pumpkin (Cucurbita pepo)/watermelon mosaic virus/inheritance of resistance/SSR分类
农业科技引用本文复制引用
王冬杰,葛宇,刘超,李雪,徐文龙,崔崇士,屈淑平..美洲南瓜（Cucurbit pepo）WMV 2抗性遗传及分子标记研究[J].河南农业科学,2014,(1):79-83,5.基金项目
公益性行业（农业）科研专项经费项目（201303112）；东北农业大学博士启动基金项目 （）
