河南农业科学Issue(1)：102-106,5.
中国莲小分子热激蛋白 HSP17．5基因克隆及表达分析
Cloning and Expression Analysis of Small Heat Shock Protein Gene HSP17.5 in Sacred Lotus
摘要
Abstract
A full length cDNA of small heat shock protein gene HSP17 .5 was isolated from sa-cred lotus by RACE method .The ORF of HSP17 .5 was 471 bp ,encodes 156 amino acid resi-dues .The deduced molecular weight and PI was 17 .5 kD and 5 .94 ,respectively .The semi-quanti-tative RT PCR result indicated that HSP17 .5 was expressed in embryonal axis during all the development period after pollination ,and the expression level was in“descent-recuperation”pat-tern .The HSP17 .5 was lowly expressed in cotyledon at 10 days after pollination ,and then disap-peared .HSP17 .5 may play an important role in seed germination and early seedling morphogenesis .关键词
中国莲/热激蛋白17.5/半定量RTPCR/基因克隆Key words
sacred lotus/HSP17 .5/semi-quantitative RT-PCR/gene cloning分类
农业科技引用本文复制引用
齐麟,李军,向志明,余英才..中国莲小分子热激蛋白 HSP17．5基因克隆及表达分析[J].河南农业科学,2014,(1):102-106,5.基金项目
公安部科技创新项目（2012YYCXTDGZ139）；江西省教育厅科学技术研究项目 （）
