河南农业科学Issue(1)：116-119,4.
光强对悬浮培养下铁皮石斛原球茎生长的影响
Effect of Light Intensity on Protocorm-like Bodies Grow th of Dendrobium candidum under Condition of Suspension Culture
摘要
Abstract
The protocorm-like bodies(PLBs) in uniform growth and dark green color were sampled to investigate the effect of light intensity on the grow th of PLBs under the condition of suspension culture ,the light intensity was set as 1 000 lx ,2 000 lx and 3 000 lx under 12 h light/12 h dark photoperiod condition ,respectively ;the shaking speed and temperature were 70 r/min and (24 ± 1) ℃ ;the fresh biomass ,content of polysaccharides and chlorophyll ,the ratio of chlorophyll a to chlorophyll b were examined every 7 days .The results showed that when the light intensity was 2 000 lx ,the growth performance of PLBs was good under the condition of liquid culture ,the fresh biomass ,content of polysaccharide (23 .4% ) and total chlorophyll in PLBs were the maxi-mum ;when the light intensity was weaker ,the ratio of chlorophyll a to chlorophyll b was lower , which was consistent with that of Dendrobium candidum seedlings under inhabitant environ-ment .The above results suggested that it might be feasible to propagate PLBs of Dendrobium candidum by suspension culture with certain light intensity under certain photoperiod condition .关键词
铁皮石斛/原球茎/光照/叶绿素Key words
Dendrobium candidum/protocorm-like bodies/light intensity/chlorophyll分类
农业科技引用本文复制引用
杨小兵,王增利,史昊,张宗申,金朝霞..光强对悬浮培养下铁皮石斛原球茎生长的影响[J].河南农业科学,2014,(1):116-119,4.基金项目
国家自然科学基金项目（31070164）；人事部博士后科学基金项目 （）
