河南农业科学Issue(1)：131-135,5.
鸡蛋内黏菌素类药物残留量检测方法的建立
Determination of Colistions Residues in Eggs by High Performance Liquid Chromatography-tandem M ass Spectrometry
摘要
Abstract
To develop a sensitive liquid chromatography tandem mass spectrometric(LC-MS/MS) method for colistin A ,colistin B and polymyxin B inbovine egg samples ,chromatographic condi-tion ,spectrometric condition and the fortreatment of samples were optimized .Egg samples were extracted with 5% Perchloric acid ,and deproteinized with aether ,and purified with Strata-X .The three colistin antibiotics in the extract were separated on a reversed phase using a gradient elution program of 2 g/L formic acid solution (A) and 0 .1% formic acid aqueous solution (B) .Using LC-MS/MS(ESI) with multiple reaction monitoring (MRM ) ,identification of the major components of the three kinds of colistin antibiotics was performed based upon the intensities of mass frag-ments from the respective doubly or triply charged precursor ions .The recoveries were 75 .6% -106 .9% for the three colistins at spiked levels of 25-200 μg/kg ,and the relative standard devia-tion was less than 20% .The limit of detection for the three colistins antibiotics was 0 .11-0 .26μg/kg .The method is suitable for quantitative and qualitative analysis of colistin antibiotics in a sufficient number of samples with simplicity ,sensitivity and repeatability ,and which could meet the request of colistin antibiotic residue testing at home and abroad .关键词
高效液相色谱/三重四级杆串联质谱仪/黏菌素类/残留/鸡蛋Key words
high performance liquid chromatography-tandem mass spectrometry/colistin antibio-tic/residue/egg分类
农业科技引用本文复制引用
曾建勇,刘红,梁雪琪,李震源..鸡蛋内黏菌素类药物残留量检测方法的建立[J].河南农业科学,2014,(1):131-135,5.基金项目
中山市科技计划项目 （）
