中医药导报Issue(10)：94-95,96,3.
HPLC法测定桐叶中齐墩果酸和熊果酸的含量
Determination of Oleanolic Acid and Ursolic Acid in Paulownia Leaves by HPLC
佘永红1
作者信息
- 1. 湘西自治州食品药品检验所，湖南 吉首 416000
- 折叠
摘要
Abstract
Objective:To establish a method for the determination of oleanolic acid and ursolic acid in Paulownia Leaves. Methods:HPLC was applied, and column was Promosil C18(4.6 mm×250 mm, 5 μm); mobile phase was acetonitrile- methanol -water-phosphoric acid (66∶12∶22∶0.02);flow rate was 1.0 mL·min -1; the detection wavelength was 210 nm; column temperature was 25 ℃; the injection volume was 20 μL. Results:Oleanolic acid in the range of 0.1296-1.5504 μg showed good linear relationship (r=0.9999),and the average recovery was 100.1%; RSD% was 3.4% (n=6). Ursolic acid in the range of 0.2428-2.9136 μg showed good linear relationship (r =0.9999), and the average recovery was 99.3% ; RSD% was 2.2% (n =6). Conclusion: The method is simple, accurate, reproducible,and can be used for Paulownia Leaves quality control.关键词
桐叶/齐墩果酸/熊果酸/HPLC法Key words
Paulownia Leaves/HPLC/Oleanolic acid/Ursolic acid/Content determination分类
医药卫生引用本文复制引用
佘永红..HPLC法测定桐叶中齐墩果酸和熊果酸的含量[J].中医药导报,2013,(10):94-95,96,3.
