华西口腔医学杂志Issue(6)：619-623,5.DOI:10.7518/hxkq.2013.06.018
不同龋敏感儿童菌斑中变异链球菌数量及菌群比例的定量分析
Quantitative analysis of Streptococcus mutans and its proportion in the dental plaque of different caries-susceptible children
赵河川 1陈霄迟 2徐韬3
作者信息
- 1. 重庆医科大学附属口腔医院预防科·口腔疾病与生物医学重庆市重点实验室，重庆 400015
- 2. 北京大学口腔医学院·口腔医院预防教研室
- 3. 北京大学口腔医学院·口腔医院预防教研室
- 折叠
摘要
Abstract
Objective To determine the quantity of Streptococcus mutans (S. mutans) and the ratio of S. mutans to total bacteria in the dental plaque of different caries-susceptible children. Methods Dental plaque samples from 26 children aged 3 years old to 4 years old were collected. The quantities of S. mutans and total bacteria were determined by TaqMan real-time fluorescence quantitative polymerase chain reaction (PCR). The ratio of S. mutans to total bacteria in children with and without caries was calculated and statistically analyzed. Results In children with and without caries, the quantities of S. mutans were 1.33×105 and 1.16×103 CFU·mg-1, the total bacteria were 7.17×107 and 1.01×108 CFU·mg-1, and the ratios of S. mutans to total bacteria were 0.058 6 and 0.018 6, respectively. Significant differences were observed in the quantities of S. mutans and the total bacteria as well as in the ratios of S. mutans to total bacteria of the two groups (P=0.033, 0.418, 0.008). Conclusion The quantities of total bacteria of the two groups show negligible difference. However, the quantity of S. mutans and the ratio of S. mutans to total bacteria in caries-susceptible children are higher than those in caries-free children. Therefore, the ratios of S. mutans to total bacteria in plaque are closely associated with the prevalence of dental caries in children.关键词
龋齿/乳牙/牙菌斑/变异链球菌/实时荧光定量聚合酶链反应Key words
dental caries/deciduous tooth/dental plaque/Streptococcus mutans/real-time fluorescence quantita-tive polymerase chain reaction分类
医药卫生引用本文复制引用
赵河川,陈霄迟,徐韬..不同龋敏感儿童菌斑中变异链球菌数量及菌群比例的定量分析[J].华西口腔医学杂志,2013,(6):619-623,5.
