华西口腔医学杂志Issue(6)：628-632,5.DOI:10.7518/hxkq.2013.06.020
雌激素对人胚髁突软骨细胞增殖分化的影响
Effects of estrogen on the proliferation and differentiation of human embryo mandibular condylar chondrocytes
摘要
Abstract
Objective To determine the effects of different estrogen concentrations on the proliferation and differentiation of human embryo mandibular condylar chondrocytes (MCCs). Methods In vitro-cultured MCCs were incubated and treated with estrogen at different concentrations. The MCCs were identified by toluidine blue and type Ⅱ collagen immunocytoche-mical methods, and cell proliferation was monitored using methyl thiazolyl tetrazolium assay. Results The addition of 10-12 or 10-6 mol·L-1 exogenous estrogen inhibited the growth of MCCs, whereas addition of 10-10 or 10-8 mol·L-1 exogenous estrogen promoted growth. These estrogen effects were time-dependent. Conclusion Estrogen can affect cellular MCCs proliferation and differentiation in a biphasic manner depending on the concentration. Estrogen may be vital in the maintenance of normal functions of the temporomandibular joint (TMJ) and may be involved in the progression of certain TMJ diseases.关键词
髁突软骨细胞/雌激素/增殖/人胚Key words
mandibular condylar chondrocytes/estrogen/proliferation/human embryo分类
生物科学引用本文复制引用
庞磊,张跃蓉,刘忠林..雌激素对人胚髁突软骨细胞增殖分化的影响[J].华西口腔医学杂志,2013,(6):628-632,5.基金项目
贵州省科学技术基金资助项目[黔科合J字（2010）2170号] （）
