华西口腔医学杂志Issue(2)：171-176,6.DOI:10.7518/hxkq.2014.02.015
Er：YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究
Effect of Er：YAG laser on the dynamic changes in subgingival flora in type 2 diabetic patients with periodontitis
摘要
Abstract
Objective To observe the changes in subgingival microflora before and after Er: YAG laser treatment on dia-betic patients with periodontitis, and to compare with the subgingival microflora of chronic periodontitis. Methods Subgingival plaque of 13 pairs of teeth (26 sites) was selected from type 2 diabetic patients at pretreatment, one month post-treatment, and three months post-treatment. Subgingival plaque was also obtained from 11 cases of moderate to severe chronic periodon-titis with similar severity of periodontitis. The DNA of the subgingival plaque samples was extracted. Whole bacterial 16S rDNA gene fragments separated by denaturing gradient gel electrophoresis. Specific DNA bands were then chosen for retrieval and sequencing. Results The gene sequencing results of the special DNA bands of subgingival plaque samples show that the pathogenic bacteria of both diabetic periodontitis and simple chronic periodontitis were Prevotella intermedia and Tannerella forsythia, respectively. The composition of the subgingival microflora before and after laser treatment changed. Some DNA bands, including that of Tannerella forsythia, disappeared or weakened one month after treatment. A new strip appeared, which belonged to Actinomyces sp. Conclusion The profiles of the subgingival microflora changed after treatment, and one month was indicated as an important stage. Er: YAG laser may have an important function in delaying microflora recolonization.关键词
Er：YAG激光/2型糖尿病/慢性牙周炎/龈下菌群/变性梯度凝胶电泳Key words
Er：YAG laser/type 2 diabetic/chronic periodontitis/subgingival plaque/denaturing gradient gel elec-trophoresis分类
医药卫生引用本文复制引用
郭斌,梁倩倩,吕岩,付敏,陈媛媛,袁一芳,李颖超..Er：YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究[J].华西口腔医学杂志,2014,(2):171-176,6.基金项目
国家自然科学基金资助项目（81271145）；四川省科技支撑计划基金资助项目 （）
