海洋科学Issue(9)：37-42,6.
海绵动物保存及DNA提取方法的研究
Comparison of samples preservation and DNA extraction of sponges
摘要
Abstract
In order to build the optional method of sponge preservation and a safe, fast and effective genomic DNA extraction method, different ways of DNA extraction and preservation were studied in this paper. Two different species, Mycale sp. and Leucosoleniidae sp., and four different ways of preservation including −20℃ freezing, ethanol fixation, air drying and ethanol fixation then drying were tested,. Three methods for DNA extraction in-cluding cetyltrimethylammonium bromide (CTAB), Phenol - Chloroform and high salt extraction were examined. The results show that each method can obtain high quality genomic DNA. But considering economical, enviromen-tal friendly and convenient factors, the method of high salt extraction was the best one among the three methods of DNA extraction. The amount and purity of DNA isolated from ethanol fixed specimens were the best. In a word, the ethanol fixed is the best method of sponge preservation.关键词
海绵/DNA提取/保存Key words
Sponge/genomic DNA extraction/preservation分类
生物科学引用本文复制引用
龚琳,欧徽龙,王德祥,柯才焕,李文宇..海绵动物保存及DNA提取方法的研究[J].海洋科学,2013,(9):37-42,6.基金项目
中央高校基本科研业务费专项资金资助项目(2010121034) （2010121034）
福建省自然科学基金资助项目(2011J01245) （2011J01245）
