海洋科学Issue(12)：1-9,9.
刺参PDRG基因全长克隆及其夏眠期间在消化道中的表达特征
Cloning and characterization of PDRG gene from sea cucum-ber Apostichopus japonicus and the expression in intestine during aestivation
摘要
Abstract
Sea cucumber (Apostichopus japonicus)undergoes an inactive phase “aestivation” as an important sur-vival strategy in high-temperature environments. During aestivation, the tissue of A. japonicus especially the intes-tine was degenerated. PDRG (p53 and DNA damage-regulated gene) was a novel gene which was found to facilitate the UV-induced cell killing and be involved in apoptosis. To investigate the gene expression of PDRG and its func-tion in tissue regression during the aestivation of A. japonicus, the PDRG was cloned, characterized and quantified in this study. The full-length cDNA sequence (1122bp) of PDRG gene from A. japonicus was cloned by SMART RACE. It consists of a 127 bp 5’ UTR (untranslated region), a 581 bp 3’ TUR and a 414 bp ORF (open reading frame). The deduced protein is composed of 127 amino acids with a molecular weight of 16.1 kDa, and its isoelec-tric point is 7.83. Sea cucumbers were induced to aestivation at temperature of 25℃ to investigate the transcrip-tional variation of PDRG during the intestine regression of aestivating A. japonicus. The intestines were sampled, at 0, 5, 10, 20 and 40 d after the temperature reached 25℃, and then analyzed using real-time PCR for PDRG gene quantification. During the thermal inducing aestivation experiment, the mRNA level of PDRG was increased to 2.49 fold at 5 d sampling time and 1.51 fold at 10 d compared to the control. No significant variation of PDRG expres-sion was observed at other sampling during the experiment. It was shown that the increase of PDRG gene expres-sion was closely associated with the regression of intestine during aestivating of A. japonicus. In summary, the re-sults of this study indicate that the PDRG has potential function in facilitating tissue regression during aestivation, which has provided a reference to explore the function of PDRG gene in animal organs degeneration.关键词
刺参(Apostichopus japonicas)/PDRG/夏眠/器官退化/实时定量PCRKey words
Apostichopus japonicus (Selenka)/PDRG/aestivation/tissue regression/Real-time PCR分类
农业科技引用本文复制引用
王天明,杨红生..刺参PDRG基因全长克隆及其夏眠期间在消化道中的表达特征[J].海洋科学,2013,(12):1-9,9.基金项目
国家“863”高技术研究发展计划项目(2006AA10A411) （2006AA10A411）
山东省农业良种工程课题“速生抗病耐高温刺参良种选育” （）
