海洋科学Issue(12)：10-16,7.
漠斑牙鲆养殖群体微卫星遗传多样性的初步分析
Primary analysis on genetic diversity in cultured stocks of Southern flounder(Paralichthys lethostigma) using microsat-ellite DNA marker
摘要
Abstract
In this study, the genetic variation and genetic structure of the first filial generation (Jiaonan cultured stock) and the third filial generation (Nantong cultured stock) of Paralichthys lethostigma were investigated using microsatellite DNA techniques. Of the 14 microsatellite DNA markers, 11 loci were polymorphic. A total of 70 al-leles (42 effective alleles) were detected at the 11 loci in the total populations. The number of alleles per polymor-phic loci ranged from 2 to 14 with an average of 7.3, the average observed and expected heterozygosities of the 11 polymorphic loci were 0.36 and 0.6, respectively. The Shannon’s index of Jiaonan and Nantong populations was 1.18 and 1.07, and the Nei’s index of the two populations was 0.56 and 0.51, respectively. The results showed that the genetic diversity of the Jiaonan population was higher than that of the Nantong population. The genetic similar-ity coefficient and the genetic distance between the two populations was 0.8432 and 0.171, respectively. The value of the Fst (0.1246) indicated that the genetic differentiation between the two populations was moderate. It can be concluded that the genetic diversity of the southern flounder population degenerated dramatically and genetic structure had differentiated significantly after several generations of cultivation in China. Thus, the scientific and reasonable broodstocks mating and seedling production strategies should be carried out in the future to maintain the genetic diversity level of Chinese farmed population of southern flounder. Moreover, selective breeding program should be in process soon in order to ensure sustainable development of farming industry of southern flounder in China.关键词
漠斑牙鲆(Paralichthys lethostigma)/养殖群体/微卫星/遗传多样性Key words
Paralichthys lethostigma/cultured stocks/microsatellite DNA marker/genetic diversity分类
生物科学引用本文复制引用
覃盼,廖梅杰,徐永江,潘传燕,李彬,王印庚,张正..漠斑牙鲆养殖群体微卫星遗传多样性的初步分析[J].海洋科学,2013,(12):10-16,7.基金项目
国家科技支撑计划课题(2012BAD17B03) （2012BAD17B03）
国家鲆鲽类产业技术体系(CARS-50) （CARS-50）
公益性行业(农业)科研专项经费项目(nyhyzx07-046-鲆鲽) （农业）
