海洋科学Issue(12)：34-39,6.
泡叶藻及海带藻渣中岩藻聚糖硫酸酯的提取及其抗氧化活性
Ultrasound assisted extraction and in vitro antioxidant activ-ity analysis of fucoidans from Ascophyllum mackaii and Laminaria japonica residue
摘要
Abstract
Ultrasound assisted extraction was used to extract fucoidans from seaweed residue and the regression model was established by uniform design in order to optimize extraction conditions. The physicochemical proper-ties and molecular weights of fucoidans from Laminaria japonica and Ascophyllum mackaii residue (LF and AF) were analyzed using high performance gel filtration chromatography (HPGFC) and their antioxidant activities were analyzed using fenton reaction and pyrogallol autoxidation assays. The results showed that the optimal condition was:ultrasound time 70 minutes, hot water extraction time 2 h, extraction temperature 90℃ and the ratio of solu-tion to algae power was 80 (mL:g). Under this condition, the extraction rates of LF and AF were 4.49%and 3.69%, respectively. Molecular weights of LF and AF were 142 kDa and 136 kDa, respectively. Both LF and AF had strong scavenging capability on hydroxyl radicals and superoxide radicals, and had excellent reducing power. Moreover, LF exhibited a stronger in vitro antioxidant activity than NF.关键词
海藻渣/岩藻聚糖硫酸酯/抗氧化活性/超声波辅助提取/理化性质Key words
Ascophyllum mackaii and Laminaria japonica residue/fucoidan/antioxidant activity/ultrasound assisted extraction/physicochemical property分类
生物科学引用本文复制引用
刘旭,曲桂燕,周裔彬,袁毅,韩丽君..泡叶藻及海带藻渣中岩藻聚糖硫酸酯的提取及其抗氧化活性[J].海洋科学,2013,(12):34-39,6.基金项目
海洋公益性行业科研专项(201105029-9) （201105029-9）
