海洋科学Issue(12)：40-46,7.
饲料中铜离子对斑节对虾血细胞组成、活性和免疫功能的影响
Effects of dietary copper on subpopulation composition, vi-ability and immune functions of haemocytes in black tiger-shrimp (Penaeus monodon)
摘要
Abstract
The black tigershrimps (Penaeus monodon) were fed with diets supplemented copper at different con-centrations (0, 10, 25, 40, 55 and 110 mg/kg) for 8-week, and the total haemocyte count (THC), percentage of cells contained granular, apoptotic cell ratio, reactive oxygen species (ROS) production and esterase activity were ana-lyzed. The results showed that THC, apoptotic cell ratio, ROS production and esterase activity were not affected by 10~55 mg/kg supplemental Cu, compared to the control group. Percentage of cells contained granular was signifi-cantly increased when the shrimpswere fed with diets containing 10~55 mg/kg supplemental Cu. In shrimps fed with diets containing 110 mg/kg supplemental Cu, the ROS production in haemocytes was significantly increased, and the apoptotic cell ratio was higher than that of the shrimps fed with diets supplemented with 25 and 55 mg/kg Cu, while THC was lower than that of the shrimps fed with diets supplemented with 55 mg/kg Cu, and the esterase activity was lower than that of the shrimps fed with diets supplemented with 25 mg/kg Cu. These results indicate that adequatedietary Cu concentration could increase the number of cells contained granular. Excess supplemental Cu (110 mg/kg) will induce the production of ROS in haemocytes, and then induce apoptosis and THC decreaseby oxidative damage.In conclusion, the adequate supplemental Cu concentration in diet for haemocyte functions in P. monodon is 25~55 mg/kg.关键词
铜/斑节对虾(Penaeus monodon)/血细胞/活性/免疫Key words
copper/Penaeus monodon/haemocytes/viability/immunity分类
农业科技引用本文复制引用
冼健安,王安利..饲料中铜离子对斑节对虾血细胞组成、活性和免疫功能的影响[J].海洋科学,2013,(12):40-46,7.基金项目
国家自然科学基金资助项目(30671628和31302164) （30671628和31302164）
广东省自然科学基金重点项目(S2011020003256和S2012040008093) （S2011020003256和S2012040008093）
中国博士后科学基金资助项目(2012M511829) （2012M511829）
广东省教育部产学研结合资助项目(2011B090500009) （2011B090500009）
广东省科技计划项目(2012B02030-7004) （2012B02030-7004）
广东省重点实验室建设项目(2009A060800019) （2009A060800019）
