激光生物学报Issue(1)：59-64,6.DOI:10.3969/j.issn.1007-7146.2014.01.012
胶质瘤细胞中miR-223对PAX6基因3′非翻译区的调控作用
The Role of miR-223 on PAX6 Gene 3′-Untranslated Region in Glioblastoma Cells
摘要
Abstract
Objective:To identify the regulation of miR-223 to PAX6 gene 3′-UTR in glioblastoma cells.Methods:Bioinformatics software was applied to predict the PAX6 3′-UTR targeting miRNAs.The wild type and mutation type of PAX6 gene 3′-UTR luciferase vectors were constructed respectively.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and miR-223 mimics into U25 1 cells. Results:Bioinformatics software prediction results showed that PAX6 may be a target of miR-223.The luciferase assay re-vealed that miR-223 mimics could significantly decrease the luciferase activity of wild type PAX6 3′-UTR plasmid,com-pared with the wild type and mutation plasmid transfection group,respectively.Conclusion:miR-223 could negatively target and regulate the activity of PAX6 gene 3′-UTR.关键词
胶质瘤/microRNA/miR-223/PAX6/3′-非翻译区Key words
glioblastoma/microRNA/miR-223/PAX6/3′-UTR分类
医药卫生引用本文复制引用
罗奇志,邬力祥,文芳,韩仰,黄柏胜..胶质瘤细胞中miR-223对PAX6基因3′非翻译区的调控作用[J].激光生物学报,2014,(1):59-64,6.基金项目
国家自然科学基金 （）
