激光生物学报Issue(1)：65-70,6.DOI:10.3969/j.issn.1007-7146.2014.01.013
AtNDPK2基因转化敖汉苜蓿及其耐盐性分析
Transgenic Alfalfa with AtNDPK2 and Salt Tolerance Analysis Plants
摘要
Abstract
Nucleoside double phosphate kinase (NDPKs)belongs to the family of protein kinases,participating in physiological and biochemical processes in plants,playing an important role in the process of the abiotic stress.Transfor-mation of alfalfa NDPK2 gene would improve the resistance of alfalfa to play an important significance in reverse.This research was based on alfalfa hypocotyls regeneration system of “Aohan”,mediated by Agrobacterium tumefaciens to transform AtNDPK2 gene driven by SWPA2 into Alfalfa hypocotyls,get resistant plants.The result of PCR preliminary analysis showed that the gene had been integrated into the alfalfa genome of“Aohan”,positive plant transformation rate was 23.3%.The results on testing of transgenic plant SOD,POD activity and membrane relative conductivity and MDA under different concentrations of NaCl stress,showed that SOD,POD enzyme activity of transgenic plants under 0.4%~1 .0%NaCl stress was significantly higher than that of control;The relative conductivity and MDA content was signifi-cantly lower than that of control,further showed that AtNDPK2 cDNA increased the Salt-tolerance of“Aohan”alfalfa.关键词
NDPK2/转化体系/盐胁迫Key words
NDPK2/transformation system/salt stress分类
生物科学引用本文复制引用
王丽娜,王丽艳,刘景文,郭永霞,金勋,芮海英..AtNDPK2基因转化敖汉苜蓿及其耐盐性分析[J].激光生物学报,2014,(1):65-70,6.基金项目
黑龙江农垦总局转NDPK2基因提高苜蓿抗逆性新种质资源的研究2010-2013 （）
