激光生物学报Issue(1)：71-76,6.DOI:10.3969/j.issn.1007-7146.2014.01.014
小麦高分子量麦谷蛋白亚基基因 Dx5的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of Wheat HMW-GS Gene Dx5
摘要
Abstract
The content of wheat high molecular weight glutenin in seeds is closely related to wheat quality.A pair of specific primer was designed for wheat high molecular weight glutenin subunit gene Dx5 according to the results of Blast search and bioinformatics analysis.The genomic DNA from high-quality wheat Jimai 20 was used as template,after PCR cloning,a 2 619 bp DNA sequence was obtained through sequencing.Bioinformatics analysis revealed that the open reading frame (ORF)was 2 520 bp long and coded for a polypeptide of 839 amino acids.The polypeptide shares up to 99% identity with Dx5 protein from GenBank database and contains a conserved domain of high molecular weight glute-nin subunit.The DNA sequence was designated as JMDx5 and submitted to GenBank database.Its accession number is KJ144185.This provided a foundation for the further study of the expression mechanism and the use for improvement of wheat quality.关键词
小麦/高分子量麦谷蛋白亚基/Dx5/生物信息学分析Key words
wheat/high molecular weight glutenin subunit/Dx5/bioinformatics analysis分类
农业科技引用本文复制引用
黄明亮,黄志刚,徐颖莹,刘清,唐蛟,李合松..小麦高分子量麦谷蛋白亚基基因 Dx5的克隆与生物信息学分析[J].激光生物学报,2014,(1):71-76,6.基金项目
国家自然科学基金（30900099）；湖南省高校创新平台开放基金项目 （）
