井冈山大学学报（自然科学版）Issue(5)：90-92,3.DOI:10.3969/j.issn.1674-8085.2013.05.020
供精个体和异种动物精浆对小鼠精子介导转染外源基因效率影响的研究
THE EFFECT OF SPERM DONOR AND HETEROGENEOUS SEMINAL PLASMA ON THE EFFICIENCY OF SPERM TRANSFECTING EXOGENOIUS DNA BY SPERM-MEDIATED GENE TRANSFER IN MICE
孙新明1
作者信息
- 1. 井冈山大学医学院，江西，吉安 343000
- 折叠
摘要
Abstract
Objective: The effect of individual and heterogeneous seminal plasma on the efficiency of sperm transfecting exogenoius DNA were investigated to explore the effecting factors of sperms used as carriers of foreign gene for generating transgenic mice. Methods:The sperms were taken from the vas deferens and ductus epididymidis of mature mice, donors of sperm, seminal plasma of different types of animal as factors, and the transfection efficiency was detected and compared by DIG-labeled mono-cloned antibody. Results:The positive transfection rates of sperms of different donors were different, but the difference was significant among some individuals (P < 0.01), not among others. The transfection rates of sperm could be significantly decreased by foreign seminal plasma added in co-incubating buffer system (The transfection rates of experimental group and control group were(8.6 ± 1.7) % vs(51.3 ± 5.3) %, respectively, P < 0.01). Conclusion: Sperm donor and heterogeneous seminal plasma can influence the efficiency of sperm transfecting exogenoius DNA in mice.关键词
精子介导基因转染/供精个体/异种动物精浆/小鼠Key words
sperm-mediated gene transfer/sperm donor/heterogeneous seminal plasma/mice分类
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孙新明..供精个体和异种动物精浆对小鼠精子介导转染外源基因效率影响的研究[J].井冈山大学学报（自然科学版）,2013,(5):90-92,3.
