江汉大学学报（自然科学版）Issue(6)：66-71,6.
富勒烯（C60）对RNA逆转录的影响
Influence of Fullerene(C60)on RNA Reverse Transcription
摘要
Abstract
Objective:To elucidate the effects of fullerene(C60)on reverse transcription(RT). Methods:Template mRNA was extracted from the liver of zebrafish,synthesised cDNA with M-MLV reverse transcriptase. Different concentrations of C60 were added into the RT reaction solu-tion,to study the impact of C60 on M-MLV reverse transcriptase and RNA conformation respectively. Results:The results showed that 100 μg/mL of C60 could significantly inhibit the RT reaction,and this effect was related to the C60 concentration. The RT inhibition of C60 could be reversed by increas-ing the amount of M-MLV reverse transcriptase in the RT reaction solution. After its co-incubation with RNA in 37℃ for 12 h without light,C60 can specifically degraded 28S rRNA in the concentra-tion-dependent manner. Conclusion:These results demonstrate that C60 could not only inhibit the activity of M-MLV reverse transcriptase,it also has damaging effect on the RNA. C60 may have po-tential genetics toxicity.关键词
富勒烯(C60)/逆转录/RNA/M-MLV逆转录酶Key words
fullerene(C60)/reverse transcription/RNA/M-MLV reverse transcriptase分类
化学化工引用本文复制引用
李佳,林燕,陈敏杰,张捷,李正,梁勇..富勒烯（C60）对RNA逆转录的影响[J].江汉大学学报（自然科学版）,2013,(6):66-71,6.基金项目
国家自然科学基金 （）
