江汉大学学报（自然科学版）Issue(6)：100-104,5.
藜豆种质资源遗传多样性的ISSR分子标记分析
Genetic Diversity Analysis of Velvet Bean Germplasm Resources with ISSR Markers
摘要
Abstract
Constructed the fingerprint of 9 velvet bean (Stizolobium cochinchinensis) germ-plasm resources and analyse their genetic diversity with ISSR markers. The results indicated that 8 primers with strong stability and repeatability were screened from 35 primers and carried out PCR amplification. 64 bands were amplified from 9 velvet bean varieties,among which 50 were polymor-phic,and the polymorphic rate was up to 78.1%. Using 12 specific bands in the 3 polymorphic ISSR primers(UBC889、UBC812、UBC825),the 9 velvet bean germplasm resources could be differentiat-ed. The genetic similarity coefficients of velvet bean germplasm ranged from 0. 47 to 0. 97. Nine vel-vet bean varieties were divided into 3 groups by UPGMA cluster analysis with the similarity coeffi-cient of 0. 73. The phylogenetic relationship of velvet beans are partly related to the geological sourc-es of the germplasm.关键词
藜豆/种质资源/遗传多样性/ISSR/相似系数/聚类分析Key words
velvet bean/germplasm resources/genetic diversity/ISSR/similarity coeffi-cient/cluster analysis分类
农业科技引用本文复制引用
张凤银,戴希刚,潘磊..藜豆种质资源遗传多样性的ISSR分子标记分析[J].江汉大学学报（自然科学版）,2013,(6):100-104,5.基金项目
湖北省豆类（蔬菜）植物工程技术研究中心开放基金项目（2012-01）；武汉市科技攻关计划项目（201120822281）；武汉市科技攻关计划项目 （）
