江汉大学学报（自然科学版）Issue(1)：55-58,4.
SPE-HPLC法检测食品中多种痕量雌激素
Determination of Trace Estrogens in Food by SPE-HPLC
摘要
Abstract
The residues of Ethinylestradiol,Diethylstilbestrol,4-Nonylphenol and Estradiol ben-zoate in food were determined simultaneously by SPE-HPLC. The samples were ultrasonic extract-ed with acetonitrile,centrifuged,concentrated and purified by C18 solid phase extraction column,an-alyzed with the HPLC method. This study optimized the conditions of SPE,and four components were well separated. The detection limit of the method was ranged from 6 to 12μg/kg. The recovery rates were 80. 3%~107. 8%. The calibration curves were linear with correlation coefficient ranged from 0.998 8 to 0.999 8. The relative standard deviations were 0. 124%~1. 72%. This method has high sensitivity,fine separability,simple sample preparation,and can reduce the inteference of im-purities,which is satisfied with the estrogen analysis.关键词
固相萃取/高效液相色谱法/痕量雌激素/食品检测Key words
solid phase extraction/HPLC/trace estrogen/food detection分类
轻工纺织引用本文复制引用
黄璐,张勇民,郑琦..SPE-HPLC法检测食品中多种痕量雌激素[J].江汉大学学报（自然科学版）,2014,(1):55-58,4.基金项目
湖北省科技厅项目（2011CDC130）；武汉市科技局项目 （）
