吉林大学学报（理学版）Issue(2)：364-370,7.DOI:10.13413/j.cnki.jdxblxb.2014.02.40
多功能淀粉酶OPMA在枯草杆菌中的分泌表达及发酵条件优化
Secretory Expression of the Multifunctional Amylase OPMA in Bacillus subtilis and the Optimization of Fermentation Conditions
摘要
Abstract
The gene encoding multifunctional amylases OPMA-N and OPMA-G were cloned into expression vector pMA5,and the secretory expression of multifunctional amylases OPMA-N and OPMA-G were achieved with the engineering strain pMA5-OPMA-N and pMA5-OPMA-G. To enhance the expression level and secretion yield of OPMA-N and OPMA-G, the fermentation conditions were further optimized.The results show that the optimal fermentation conditions for the expression and secretion of OPMA-N were obtained in the culture medium (1 6%)containing 0.5%yeast extract,0.3% urea,1% MgSO 4 ,at pH=6.5 and 37 ℃ for 48 h,and for the expression and secretion of OPMA-G was obtained in the culture medium (32%)containing 1% starch,0.8% yeast extract,1% NaCl,at pH=7.0,and 37 ℃ for 36 h.Under the optimal conditions,the secretion yields of OPMA-N and OPMA-G were about 45% and 58% of the total extracellular proteins, respectively and the specific activities of the secreted OPMA-N and OPMA-G were 4.57 and 4.65 enzymology activity unit per mg.关键词
多功能淀粉酶/枯草杆菌/表达/发酵/活力Key words
multifunctional amylase/Bacillus subtilis/expression/fermentation/activity分类
生物科学引用本文复制引用
李雁飞,夏莹,梁小博,张应玖..多功能淀粉酶OPMA在枯草杆菌中的分泌表达及发酵条件优化[J].吉林大学学报（理学版）,2014,(2):364-370,7.基金项目
国家自然科学基金(批准号:31170759 （）
30870518)和吉林省发改委项目(批准号:2011003-5) （批准号:2011003-5）
