吉林大学学报（理学版）Issue(2)：371-375,5.DOI:10.13413/j.cnki.jdxblxb.2014.02.41
几种基于pCAMBIA系列多用途新型植物表达载体的改建及优化
Reconstruction and Optimization of pCAMBIA-Based Plant Expression Vectors for Multifunctional Application
摘要
Abstract
To supply the required large amounts of functional recombinant plant expression vectors,a series of general plant binary expression vectors with the CaMV35S and the rd 29A promoter mediating multiple cloning sites (MCS:SacⅠ,KpnⅠ,SmaⅠ,BamⅠ,XbaⅠ,SalⅠ,P stⅠ)were constructed successfully, on the basis of pCHF3, pCAMBIA1301, pCAMBIA3300 and pCAMBIA3301 vectors,and a set of applicative binary expression vectors utilized for subcellular localization by the fusion of MCS and eGFP with these two promoters were also constructed. Subsequently, the universal high-throughput and expense-saving gene expression system was established for the scan and functional characterization of a large number of candidate genes.关键词
植物双元表达载体/CaMV 35S/rd 29A/多克隆位点/增强型绿色荧光蛋白Key words
plant binary expression vector/CaMV 35S/rd 29A/multple clonging sites (MCS)/enhanced green fluorescent protein (eGFP)分类
生物科学引用本文复制引用
李敬涛,孙新华,余刚,贾承国,杜茜,李启云,潘洪玉..几种基于pCAMBIA系列多用途新型植物表达载体的改建及优化[J].吉林大学学报（理学版）,2014,(2):371-375,5.基金项目
国家科技支撑计划项目(批准号:2012BAD19B04)和农业部抗病虫转基因大豆新品种培育项目(批准号:2013ZX08004004) （批准号:2012BAD19B04）
