吉林农业大学学报Issue(6)：732-735,741,5.
猪肌生成抑制素单克隆抗体的制备及鉴定
Preparation and Identification of Porcine Myostatin Monoclonal Anti-body
摘要
Abstract
Balb /c mice were immunized with purified recombinant GST-MSTN protein.Hybridoma cell lines were set up by using hybridoma technique,and monoclonal antibodies were prepared.Using puri-fied C-termination function MSTN as detection antigen,titer of immuned mice serum antibody,titer of hy-bridoma culture supernatant,the type and immunoglobulin subclasses and additive index of antibody were determined with enzyme linked immunosorbent assay (ELISA).Western blot analysis was used as anti-bodies specific.The results showed that the experiment obtained 3 stable secreting anti-porcine myostatin monoclonal antibody cell line,named 3D3,1G8 and 10H1 .The titer of immuned mice serum antibody of the mice,detected by ELISA,was 1 ∶400,1 ∶800 and 1 ∶800.The ascites titer of 3 monoclonal antibody (3D3,1G8 and 10H1)were 1 ∶16 000,1 ∶32 000 and 1 ∶32 000.Western blot analysis showed that all of the monoclonal antibodies could specifically bind to recombinant MSTN protein.Immunoglobulin sub-classes of antibodies secreted by hybridoma cells displayed both in 3D3 are IgM,and 1G8 and 10H1 be-long to IgG2a.The results of three antibodies additive experiments show that 3D3,1G8 and 10H1 have different antigen recognition sites.关键词
猪/肌成生抑制素/单克隆抗体Key words
porcine/myotatin/monoclonal antibody分类
农业科技引用本文复制引用
车东升,张春,李占军,李莉,逄大欣,欧阳红生..猪肌生成抑制素单克隆抗体的制备及鉴定[J].吉林农业大学学报,2013,(6):732-735,741,5.基金项目
国家自然科学基金项目（31201763），吉林省教育厅青年基金项目 （）
