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仔猪黄痢大肠杆菌的分离鉴定与耐药分析

郑礼洲 黄冬艳 王玉来 左明开 黎家祥 吴家斌 王萍

江西农业大学学报Issue(6):1255-1260,6.
江西农业大学学报Issue(6):1255-1260,6.

仔猪黄痢大肠杆菌的分离鉴定与耐药分析

Isolation and Identification of E.Coli from the Yellow Scour of Newborn Piglets and Analysis of their Antibiotic Resistance

郑礼洲 1黄冬艳 1王玉来 2左明开 1黎家祥 1吴家斌 1王萍1

作者信息

  • 1. 江西农业大学 动物科学技术学院,江西 南昌 330045
  • 2. 江西省彭泽县黄岭乡兽医站,江西 彭泽 332700
  • 折叠

摘要

Abstract

Class1 integron is strongly associated with multidrug resistance in bacteria .In this study , the drug sensitivity of the pathogenic E.coli strains isolated from piglets to 21 antimicrobial agents was tested by the Kirby-Bauer method,and the genes of Class 1 Integron and qacE△1-sul1 were detected by PCR.The results showed that the isolates were only sensitive to chloramphenicol ,and resistant to the other 20 drugs.The isolates were positive for intI1 and qacE△1-sul1 gene,and the detect to intI1 gene cassette showed that the isolates harbored some kinds of gene sequens .The result suggests that it is critical to monitor drug resistant bacteria at the gene level .

关键词

仔猪/致病性大肠杆菌/整合子/耐药分析

Key words

piglet/Escherichia coli/integron/drug resistance analysis

分类

农业科技

引用本文复制引用

郑礼洲,黄冬艳,王玉来,左明开,黎家祥,吴家斌,王萍..仔猪黄痢大肠杆菌的分离鉴定与耐药分析[J].江西农业大学学报,2013,(6):1255-1260,6.

基金项目

江西省科技厅科技支撑计划(2009BNA0700)和江西省教育厅科学技术研究项目 ()

江西农业大学学报

OA北大核心CSCDCSTPCD

1000-2286

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