江西农业大学学报Issue(1)：68-75,8.
金银花叶 HPLC指纹图谱及最佳采收期的研究
A Study on HPLC Fingerprint Chromatogram and Optimum Harvest Time of Lonicera japonica Thunb.Leaves
摘要
Abstract
To established the fingerprint chromatogram by HPLC of Lonicera japonica leaves collected at different time and determine the optimum harvest period .The HPLC fingerprints were obtained with Chlorogenic acid as the interna1 standard and on Alltech Alltima C18 column (250 mm×4.6 mm i.d.,5μm) at 40 ℃and detected at 238 nm by a linear gradient of eluents A (acetonitrile) and B (water/phosphoric acid,100:0.4,v/v) using mobile phase at a flow rate of 0.7 mL/min,and then investigated by similarity analysis .There were significant differences in the chromatographic profiles of the samples collected in different months .The impact of harvest time on the quality of Lonicera japonica leaves should be noted and the best harvest period is July .关键词
金银花叶/高效液相色谱/指纹图谱/采收期Key words
Lonicera japonica leaves/HPLC/fingerprints/harvest分类
农业科技引用本文复制引用
杨鹏飞,熊建华,杜华英,罗秋水,吴国平..金银花叶 HPLC指纹图谱及最佳采收期的研究[J].江西农业大学学报,2014,(1):68-75,8.基金项目
国家自然科学基金项目（31060226）和江西省科技支撑项目 （）
