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HPLC法测定枣果中多酚类物质含量研究

郝婕 董金皋 王献革 李学营 鄢新民 冯建忠

江西农业学报Issue(10):103-105,3.
江西农业学报Issue(10):103-105,3.

HPLC法测定枣果中多酚类物质含量研究

Study on Determination of Polyphenols Content in Jujube Fruit by HPLC Method

郝婕 1董金皋 2王献革 1李学营 1鄢新民 1冯建忠1

作者信息

  • 1. 河北省农林科学院 石家庄果树研究所,河北 石家庄 050061
  • 2. 河北农业大学 生命科学学院,河北 保定 071001
  • 折叠

摘要

Abstract

The total content of natural polyphenols with antioxidant activity extracted from jujube fruit was tested by tartaric acid iron method.In order to determine the polyphenol species composition and the content of each polyphenol , the author separated and purified step-by-step the natural polyphenols from jujube fruit by using HPLC and other chromatographic methods .The results showed that the total content of polyphenols in jujube fruit was 10.35 mg/g・ FW by tartaric acid iron method.After separation by using thin-layer chromatography, polyamide column chromatography and HPLC methods successively , the polyphenols in jujube fruit mainly included phloroglucinol and pyrocatechol , whose content was 55.35 μg/mL, 38.41 μg/mL respectively.

关键词

HPLC/枣果/多酚/含量

Key words

HPLC/Jujube fruit/Polyphenols/Content

分类

农业科技

引用本文复制引用

郝婕,董金皋,王献革,李学营,鄢新民,冯建忠..HPLC法测定枣果中多酚类物质含量研究[J].江西农业学报,2013,(10):103-105,3.

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