江西农业学报Issue(11)：6-10,5.
棕榈科植物 SSR 分子标记反应体系的建立
Establishment of SSR-PCR Reaction System for Palm Plants
摘要
Abstract
To establish the optimal SSR-PCR reaction system for palm trees , different factors that mostly affected the amplifi-cation of SSR-PCR reaction, including DNA template, dNTPs, primers, TaqDNA and MgCl2, were investigated and optimized by setting up 5 gradients with single-fact test method in the experiment .Meanwhile , we also compared the influences of different con-centrations and different consumptions on the amplification .The results showed that the optimal SSR -PCR system was DNA tem-plate 2 μL, dNTPs 0.2 μL, primers 1.0 μL, TaqDNA 0.2 μL, and MgCl2 2.0 μL, which was more stable and suitable for the SSR analysis of palm plants .关键词
棕榈科/SSR/优化Key words
Palm tree/SSR/Optimization分类
农业科技引用本文复制引用
符海泉,杨耀东,符海瑜,万婕,马子龙,吴翼..棕榈科植物 SSR 分子标记反应体系的建立[J].江西农业学报,2013,(11):6-10,5.基金项目
海南省自然科学基金（No．312039）。 （）
