江西农业学报Issue(11)：105-107,3.
用基于邻苯二甲醛柱前衍生的高效液相色谱法测定稻米的γ-氨基丁酸含量
Determination of γ-aminobutyric Acid Content in Rice by High Performance Liquid Chromatography Based on Pre-column Derivatization with o-phthalaldehyde
摘要
Abstract
The high performance liquid chromatography ( HPLC) method based on pre-column derivatization with o -phthal-aldehyde (OPA) was established, and it was used to determine the content of γ-aminobutyric acid (GABA) in rice.The results indicated that trichloroacetic acid used as solvent had the best extract effect ;the detection limit of this method was 0.5 mg/L, and the recoveries were 79.1%~101.5%;the content of GABA in brown rice with giant embryo was significantly higher than that in brown rice with normal embryo;after germination, the accumulation of GABA content in brown rice with giant embryo was also high-er than that in brown rice with normal embryo .关键词
γ-氨基丁酸/邻苯二甲醛柱前衍生/高效液相色谱/水稻/测定Key words
γ-aminobutyric acid/Pre-column derivatization with o -phthalaldehyde/High performance liquid chromatog-raphy/Rice/Determination分类
生物科学引用本文复制引用
张标金,廖且根,张祥喜,罗林广..用基于邻苯二甲醛柱前衍生的高效液相色谱法测定稻米的γ-氨基丁酸含量[J].江西农业学报,2013,(11):105-107,3.基金项目
农业部“948”项目（2005-Z26）；农业部公益性行业（农业）专项（200803056）；江西省财政厅项目；江西省农业厅“农牧渔业科技计划”项目（Ny0811）；江西省农业科学院创新（青年）基金项目（2009青-12）。 （）
