江西农业学报Issue(2)：49-54,6.
CaKR1上游启动子分离及其表达分析
Isolation and Expressional Analysis of Upstream Promoter of Gene CaKR1
黄雪盈 1刘志钦 2贺俐 1吴杨 2何水林 3石兰平 3杨晟 1卢蓉 4王博 1申磊 2刘艳艳 1洪水秀 2梁浩1
作者信息
- 1. 福建农林大学 作物遗传育种与综合利用教育部重点实验室，福建 福州 350002
- 2. 福建农林大学 生命科学学院，福建 福州 350002
- 3. 井冈山大学 生命科学学院，江西 吉安343009
- 4. 福建农林大学 作物科学学院，福建 福州 350002
- 折叠
摘要
Abstract
In the present study , the -1594 bp upstream promoter sequence of gene CaKR1 was isolated by using genome walk-ing technology and named CaKR1p, and cis-elements such as SARE, ABRE, LTRE were found in this promoter.The promoter was fused to GUS reporter gene to generate plant expression vector .This expression vector was transformed into tobacco plants by u-sing Agrobacterium-mediated method , and the transgenic tobacco plants were acquired and confirmed .With T1 transgenic tobacco lines, we analyzed the expression of CaKR1p against the treatments of several exogenous hormones , and found that the expression of GUS reporter gene was induced significantly by exogenously -applied ABA, JA and SA, suggesting that the expression and function of CaKR1 are regulated by the signal paths of SA , ABA, JA and so on.关键词
辣椒/烟草/锚定蛋白/启动子/表达/转基因Key words
Pepper/Tobacco/Anchor protein/Promoter/Expression/Transgene分类
农业科技引用本文复制引用
黄雪盈,刘志钦,贺俐,吴杨,何水林,石兰平,杨晟,卢蓉,王博,申磊,刘艳艳,洪水秀,梁浩..CaKR1上游启动子分离及其表达分析[J].江西农业学报,2014,(2):49-54,6.
