江西农业学报Issue(3):16-17,21,3.
宁玉草莓茎尖组培快繁技术研究
Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of Strawberry “Ningyu”
摘要
Abstract
The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.关键词
宁玉草莓/组织培养/快繁技术Key words
“Ningyu” strawberry/Tissue culture/Rapid propagation分类
农业科技引用本文复制引用
夏瑾,赵密珍,孟宪凤..宁玉草莓茎尖组培快繁技术研究[J].江西农业学报,2014,(3):16-17,21,3.基金项目
农业科技成果转化资金项目(2012GB2C100159)。 ()