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宁玉草莓茎尖组培快繁技术研究

夏瑾 赵密珍 孟宪凤

江西农业学报Issue(3):16-17,21,3.
江西农业学报Issue(3):16-17,21,3.

宁玉草莓茎尖组培快繁技术研究

Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of Strawberry “Ningyu”

夏瑾 1赵密珍 1孟宪凤1

作者信息

  • 1. 江苏省农业科学院 园艺研究所,江苏 南京 210014
  • 折叠

摘要

Abstract

The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.

关键词

宁玉草莓/组织培养/快繁技术

Key words

“Ningyu” strawberry/Tissue culture/Rapid propagation

分类

农业科技

引用本文复制引用

夏瑾,赵密珍,孟宪凤..宁玉草莓茎尖组培快繁技术研究[J].江西农业学报,2014,(3):16-17,21,3.

基金项目

农业科技成果转化资金项目(2012GB2C100159)。 ()

江西农业学报

OACSTPCD

1001-8581

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