生物灾害科学Issue(4)：399-402,416,5.DOI:10.3969/j.issn.2095-3704.2013.04.012
犬细小病毒病PCR检测方法的建立及应用
Establishment and Application of PCR Detection Methods of Canine Parvovirus
摘要
Abstract
Canine parvovirus infection is caused by canine parvovirus (CPV), and is one of the most important infectious diseases causing dog deaths. The current diagnosis of the disease mainly uses the colloidal gold rapid detection test plate method. Although this detection method is much simple and rapid, but its sensitivity is poor. Based on the analysis of CPV2 genome sequence published in the website NCBI GenBank, the author selected the gene conserved sequence of the virus VP2, then designed primers to amplify the material through positive diseases and sequencing of PCR products, and compared them with related genes published in NCBI. We found they were homologous from 99.8%to 100%, and successfully established the PCR method with specificity and high sensitivity, and the minimum was just 2.5 pg DNA template. In 28 cases of suspicious CPV case detection, this method detected 25 cases as positive, and 3 as negative, with 89.28% positive rate; the colloidal gold assay plates detected 20 cases as positive, and 8 as negative, with 71.42%positive rate. This confirmed that the PCR method was more sensitive than the colloidal gold assay test.关键词
犬细小病毒/PCR/检测方法Key words
canine parvovirus/PCR/detection methods分类
农业科技引用本文复制引用
陶明江,张祥华,孙明,余恩超,陆家卿,王萍,何后军,邬向东,戴益民..犬细小病毒病PCR检测方法的建立及应用[J].生物灾害科学,2013,(4):399-402,416,5.基金项目
江西省教育厅重点项目（GJJ10017）和江西省南昌市科技支撑项目 （）
