生物灾害科学Issue(4)：408-413,6.DOI:10.3969/j.issn.2095-3704.2013.04.014
胭脂鱼致病性嗜水气单胞菌的分离鉴定及药物敏感试验
Isolation, Identification and Antibiotic Sensitivity of Pathogenic Bacterium Aeromonas hydrophila from Chinese Sucker Myxocyprinus asiaticus
摘要
Abstract
A strain bacterium (YFYZY001) was isolated from the viscera of juvenile Chinese sucker Myxocyprinus asiaticus and identified as Aeromonas hydrophila by ATB expression automatic microbiological assay system. The strain bacterium YFYZY001 was found to have a sequence of 1 453 base pair (bp) from GenBank with accession number KF242091 by 16S rDNA sequence analysis, with similarity value of 99%with other A. hydrophila strains. A phylogenetic tree from GenBank database revealed that the strain YFYZY001 was clustered with A. hydrophila, indicating that the strain YFYZY001 was identified as A. hydrophila. An artificial infection proved that the strain YFYZY001 had pathogenicity to the examined grass carp and carried virulence factors by detection. The antibiotic sensitivity test of 22 antimicrobial agents showed that the strain YFYZY001 was sensitive to 15 antibiotics, including streptomycin.关键词
胭脂鱼/嗜水气单胞菌/分离鉴定/药物敏感性/毒力因子Key words
Myxocyprinus asiaticus/Aeromonas hydrophila/isolation and identification/antibiotic sensi tivity;virulence factor分类
农业科技引用本文复制引用
田飞焱,欧阳敏,花麒,张晓燕,刘文珍..胭脂鱼致病性嗜水气单胞菌的分离鉴定及药物敏感试验[J].生物灾害科学,2013,(4):408-413,6.基金项目
江西省水生动物疫病监测计划（2013年江西省渔业专项007号）和公益性行业（农业）科研专项（201203083（02）） （）
