解放军医学院学报Issue(12)：1259-1261,3.DOI:10.3969/j.issn.2095-5227.2013.12.019
睾酮对体外培养小鼠卵巢颗粒细胞分泌抗苗勒管激素的影响
Effect of testosterone on secretion of anti-Mullerian hormone in mouse ovarian granular cells cultured in vitro
摘要
Abstract
Objective To study the effect of testosterone on secretion of anti-Mullerian hormone (AMH) in mouse ovarian granular cells cultured in vitro.Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA.Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10-8 mmol/L, 10-7 mmol/L and 10-6 mmol/L up-regulated their secretion of AMH in a concentration- and time-dependent manner (P<0.01).Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.关键词
睾酮/颗粒细胞/抗苗勒管激素Key words
testosterone/granular cells/anti-Mullerian hormone分类
医药卫生引用本文复制引用
张颖,郑金丹,刘丽丽..睾酮对体外培养小鼠卵巢颗粒细胞分泌抗苗勒管激素的影响[J].解放军医学院学报,2013,(12):1259-1261,3.基金项目
辽宁省自然科学基金(201102134)；锦州市科学技术计划项目(12A1E35) Supported by the Liaoning Natural Science Foundation of China(201102134) （201102134）
