解放军医学院学报Issue(3)：283-285,3.DOI:10.3969/j.issn.2095-5227.2014.03.025
标本保存温度和保存时间对流式自然杀伤细胞和B细胞数量检测的影响
Effect of sample storage temperature and time on NK cells and B-lymphocytes detected by flow cytometry
周方圆 1张蕴莉1
作者信息
- 1. 辽宁医学院附属第一医院 检验科，辽宁锦州，121000
- 折叠
摘要
Abstract
Objective To study the effect of storage temperature and time on the detection of NK cells and B-lymphocytes. Methods NK cells and B-lymphocytes in peripheral blood were detected by 6-color flow cytometry. The effect of storage temperature and time on the detection of NK cells and B-lymphocytes was observed. Results No change was found in the number of NK cells and B-lymphocytes in whole blood samples stored at 18℃. The number of NK cells and B-lymphocytes decreased after stored at low or high temperature. The whole blood samples that were fixed with paraformaldehyde and stained with fluorescent antibody could be stored for 7 days. Conclusion Flow cytometry can detect NK cells and B-lymphocytes. The samples can be stored at 18℃for 96 h when it is not able to treat them in time. The treated samples can be stored at 4℃for 7 days.关键词
自然杀伤细胞/B淋巴细胞/流式细胞术Key words
natural killer cell/B-lymphocyte/flow cytometry分类
医药卫生引用本文复制引用
周方圆,张蕴莉..标本保存温度和保存时间对流式自然杀伤细胞和B细胞数量检测的影响[J].解放军医学院学报,2014,(3):283-285,3.
