检验医学与临床Issue(21)：2785-2787,3.DOI:10.3969/j.issn.1672-9455.2013.21.001
人5型腺病毒六邻体蛋白单克隆抗体的制备及鉴定
Preparation and identification of specific monoclonal antibodies against hexon of HAdV 5
摘要
Abstract
Objective To obtain the monoclonal antibody against hexon protein of human adenovirus type 5 (HAdV5) .Methods BALB/c mice were immunized with purified hexon protein ,and the spleen cells of the mice were isolated and fused with myloma cells .Four hybridoma cell strains were screened by indirect ELISA and cul-tured ,and the sensitivity and specificity were analyzed by ELISA and Western blotting test .Results These hybrido-ma cells contained specific monoclonal antibodies against hexon protein of HAdV 5 as identified by ELISA and West-ern blot .Conclusion The monoclonal antibodies against hexon protein of HAdV5 with high specificity have been successfully obtained ,and these antibodies could be useful in developing assays for early diagnosis of HAdV 5 infec-tion and also in vaccine development based on HAdV5 virus .关键词
5型腺病毒/六邻体/单克隆抗体Key words
adenovirus type 5/hexon/monoclonal antibody引用本文复制引用
潘庆军,朱学芝,叶玲..人5型腺病毒六邻体蛋白单克隆抗体的制备及鉴定[J].检验医学与临床,2013,(21):2785-2787,3.基金项目
国家自然科学基金资助项目（81202346）；广东省自然科学基金资助项目（S2012040006216）；广东省卫生厅基金资助项目（B2012284）；广东省湛江市科技攻关项目（2012C3101028）。 （）
