检验医学与临床Issue(21)：2791-2792,2795,3.DOI:10.3969/j.issn.1672-9455.2013.21.003
产ESBLs尿路感染大肠埃希菌的耐药基因型分析
Drug resistance genotyping of Escherichia coli producing extended-spectrumβ-Lactamases in urinary tract infection
摘要
Abstract
Objective To investigate the antibiotic resistance situation and genotypes of extended-spectrum β-Lactamases(ESBLs)-producing Escherichia coli(E .coli) in urinary tract infection (UTIs) .Methods The antibiotic susceptibility test was performed by Kirby-Bauer method .The ESBLs-producing E .coli was confirmed by phenotypic confirmatory test .The genotypes of ESBLs were examined by polymerase chain reaction (PCR) and nucleic acid elec-trophoresis .Results The ESBLs-producing E .coli were more sensitive to meropenem ,imipenem and cefoxitin ,but resistant to ampicillin ,piperacillin and cefotaxime .48 .6% of the ESBLs positive isolates was found carried CTX-M gene ,87 .5% of the ESBLs positive isolates was found carried TEM gene ,and no isolate was found carried SHV gene .47 .2% of the strains were found carried both the CTX-M and TEM genes .Conclusion The antibiotic resist-ance situation of the ESBLs-producing E .coli in UTIs could be very serious .CTX-M and TEM genes might be the predominant genetypes of the ESBLs-producing E .coli in UTIs .关键词
尿路感染/大肠埃希菌/超广谱β-内酰胺酶/基因型Key words
urinary tract infections/Escherichia coli/extended-spectrum β-Lactamases/genotype引用本文复制引用
彭亮,陈姬明,肖柯玲..产ESBLs尿路感染大肠埃希菌的耐药基因型分析[J].检验医学与临床,2013,(21):2791-2792,2795,3.基金项目
广东省广州医学院博士启动项目（2011C48）。 （）
