检验医学与临床Issue(21)：2807-2808,2810,3.DOI:10.3969/j.issn.1672-9455.2013.21.011
两台化学发光仪检测人生长激素的比对分析及偏倚评估
Comparative analysis and bias assessment of detecting results of human growth hormone using two chemiluminescence ap-paratus
杨方华 1邓芳梅 1孙丽芳1
作者信息
- 1. 广东省深圳市儿童医院检验科 518026
- 折叠
摘要
Abstract
Objective To explore the comparability of human growth hormone(hGH) detected by chemilumi-nescence apparatus of SIEM ENS Immulite 1000 and Beckman Coulter DXI 800 detection systems ,through methodol-ogy comparing and bias assessing .Methods According to Clinic and Laboratory Standrd Instritate (CLSI) EP9-A2 document ,at least 8 copies of different concentrations of fresh serum were collected everyday ,then we tested hGH with two detection systems for at least 5 days continuously .Every sample was detected repeatedly with obverse and reverse sequence ,then we recorded the results ,checked outlier ,observed linearity and data distribute ,and calculated correlation coefficient and linear regression equation .According to the regression equation ,we calculated hGH in the critical value of the expected bias and the expected bias of the 95% confidence interval ,and determined the bias ac-cepted or not .Results The correlation coefficient (r) of two detection systems in hGH was 0 .999 ,and the difference of mean results was negative bias .The bias was in the permissible error range ,and it was acceptable .Conclusion Methodology comparison and bias estimation of the same test items in different detection systems should be carried out regularly in order to ensure comparability and continuity of test results .关键词
生长激素/方法比对/偏倚评估Key words
grow hormone/method comparison/bias estimation引用本文复制引用
杨方华,邓芳梅,孙丽芳..两台化学发光仪检测人生长激素的比对分析及偏倚评估[J].检验医学与临床,2013,(21):2807-2808,2810,3.
