检验医学与临床Issue(22)：2978-2979,2981,3.DOI:10.3969/j.issn.1672-9455.2013.22.019
羧基磁珠吸附乙型肝炎病毒表面抗原的性能研究
Research of carboxyl magnetic beads adsorption performance of HBsAg
摘要
Abstract
Objective To lay the foundation for the application of magnetic beads in protein purification by re-searching carboxyl magnetic beads adsorption performance of HBsAg .Methods The amount of magnetic beads ,HB-sAg concentration ,adsorption temperature and the influence of adsorption time by detecting the protein amount be-fore and after adsorption were detected .Results Adsorption HBsAg capacity of magnetic beads was affected by a-mount of magnetic beads ,HBsAg concentration ,adsorption temperature and adsorption time .After magnetic beads adsorption of HBsAg whose concentration was 524 .24 μg/mL ,the adsorbed HBsAg could be eluted by 10 mmol/L NaOH ,the elution rate was 71 .02% ,and the retained biological activity was 88 .15% .Conclusion Magnetic beads with carboxyl can adsorption HBsAg ,which is expected to be used for the separation and recovery of HBsAg .It has lay the foundation for the application of magnetic beads in protein purification.关键词
磁珠/乙型肝炎病毒表面抗原/吸附Key words
magnetic beads/HBsAg/adsorption引用本文复制引用
林梅双,吴晓蔓..羧基磁珠吸附乙型肝炎病毒表面抗原的性能研究[J].检验医学与临床,2013,(22):2978-2979,2981,3.基金项目
广东省广州市属高校科研项目（10A204）。 （）
