检验医学与临床Issue(23)：3108-3109,3135,3.DOI:10.3969/j.issn.1672-9455.2013.23.010
人乳头状瘤病毒58型 L1基因序列测定及同源性分析
Sequencing and homology analysis on human papillomavirus type 58 L1 gene
摘要
Abstract
Objective To understand the structure and variation regulation of human papillomavirus (HPV ) type 58 L1 gene in Putian city ,Fujian province ,in order to provide theoretical basis for clinical treatment and preven-tion .Methods Nucleic acids rapid diversion genotyping chip(HybrMax) was used in HPV genotype .Four strains of type HPV58 positive samples was randomly selected ,then cloned and sequenced ;further results sequenced and 32 strains of HPV type 58 virus strains L1 gene nucleotide sequence in Gen Bank were used to establish homology ma-trix ,analyzed and build phylogenetic tree construction by DNAstar ,clustalx 2 and Mega5 .0 software .Results The gene of these four strains of HPV virus type 58 strains shared highly homology 96% - 98% in nucleotide with each other ,92% - 98% in protein level too ;with 1986 French line ,nucleotide homology is 83% - 86% ,the homology of a-mino acid is 84% - 89% ;with 1987 the British lines ,nucleotide homology is 53% - 54% ,the amino acid sequence is 39% - 42% ;with separation and the 2010 Hongkong ,2004 Jiangsu isolates nucleotide strains ,homology is 96% -100% ,the homology of amino acid is 92% - 99% ,the highest homology .From the phylogenetic tree diagram ,the gene background of these 4 strains of HPV58 virus in the region and that of the EV999963 ,DQ057326 ,HM639611 strains virus from Shanghai ,Hongkong were most closely .Conclusion The region type HPV58L1gene has certain regional characteristics .关键词
人乳头状瘤病毒/基因型/同源性/基因序列Key words
human papilloma virus/genotype/homology/gene sequence引用本文复制引用
涂海健,俞柳敏,李励军,陈智伟..人乳头状瘤病毒58型 L1基因序列测定及同源性分析[J].检验医学与临床,2013,(23):3108-3109,3135,3.基金项目
福建省莆田市科技立项项目［2009S09（1-2）］。 （）
