检验医学与临床Issue(4)：433-435,3.DOI:10.3969/j.issn.1672-9455.2014.04.001
人脐带和骨髓源间充质干细胞对脐血单个核细胞增殖能力的影响
Effects of human mesenchymal stem cells derived from bone marrow and umbilical cord on the proliferation of umbilical ;cord blood mononuclear cells
摘要
Abstract
Objective To explore the supportive effects of mesenchymal stem cells (MSCs)derived from hu-man umbilical cord (UCMSCs)and bone marrow (BMMSCs)on hematopoiesis in vitro.Methods MSCs were isola-ted from umbilical cord and bone marrow,and the purity of MSCs was analyzed by immunocytochemical stain.The distribution of mononuclear cell cycle was determined by flow cytometry.Half-solid methylcellulose was used to de-termine the colony forming unit-mixture (CFU-mix)of umbilical cord blood mononuclear cells.Influence of UCM-SCs and BMMSCs on cell cycle and forming ability of CFU-Mix among umbilical cord blood mononuclear cells were compared.Results UCMSCs and BMMSCs were both successfully isolated and identified.Compared with the blank control group,there were more umbilical cord blood mononuclear cells in S+G2+M phase and CFU-mix after inter-vening with UCMSCs and BMMSCs (P<0.05),but the difference between UCMSCs and BMMSCs was not signifi-cant (P>0.05).Conclusion UCMSCs and BMMSCs were successfully isolated and identified,both of which could in vitro improve the proliferation and CFU-Mix forming ability of umbilical cord blood mononuclear cells,and might be able to support hematopoiesis.关键词
脐带/骨髓/间充质干细胞/体外/脐血单个核细胞/细胞增殖Key words
umbilical cord/bone marrow/mesenchymal stem cells/in vitro/umbilical cord blood mononuclear cells/proliferation引用本文复制引用
张玉琳,王静文,于纪棉..人脐带和骨髓源间充质干细胞对脐血单个核细胞增殖能力的影响[J].检验医学与临床,2014,(4):433-435,3.基金项目
浙江省教育厅科研项目课题（Y201226068）。 （）
