检验医学与临床Issue(5)：577-579,582,4.DOI:10.3969/j.issn.1672-9455.2014.05.001
乙型肝炎病毒表面抗原特异性结合肽的筛选与鉴定
Screening and identification of HBV surface antigen specific binding peptide
摘要
Abstract
Objective To select and identify the peptide that specifically binds with HBV surface antigen (HB-sAg) .Methods HBsAg dereved from yeast was coated on milipore plate ,phage was added to screen peptide ran-domly .After four rounds of screening ,15 phage clones were selected randomly ,the phage DNA were extracted and sequenced .And their affinities were identified by the sandwich ELISA ,the specificity was identified by the does de-pendent binding test and competitive inhibition test .Results After four rounds screening ,a predominant phage clone No .3 was obtained which could comparatively strong bind with HBsAg ,and this clone displayed a peptide sequence of SSYAPYVWQPIA .HBsAg dose dependent binding test and competitive inhibition test showed that No .3 binding with HBsAg was specific .Conclusion Peptides specifically binding to HBsAg could be obtained through the screen-ing of phage display peptide library .These peptides may be used in target gene therapy of hepatitis B .It will provide experiment foundation of target gene therapy of HBV infection .关键词
乙型肝炎病毒/乙型肝炎病毒表面抗原/噬菌体展示/结合肽Key words
hepatitis B virus/HBsAg/phage display/binding peptide引用本文复制引用
孙鸣,马智勇,黄朝阳,胡斌,沈关心..乙型肝炎病毒表面抗原特异性结合肽的筛选与鉴定[J].检验医学与临床,2014,(5):577-579,582,4.基金项目
福建省自然科学基金青年项目（2011D012）。 （）
