检验医学与临床Issue(5)：583-585,588,4.DOI:10.3969/j.issn.1672-9455.2014.05.003
人细小病毒B19IgM抗体间接ELISA检测方法的初步建立
Development of an indirect enzyme liked immunosorbent assay for detecting IgM antibodies of human parvovirus B 19
摘要
Abstract
Objective Investigated prokaryotic expression and purification of structural protein VP 1 of human parvovirus B19(HPV B19) ,and detected HPV infection by an IgM-ELISA method .Methods We established and preliminarily applied an indirect ELISA method detecting IgM where the purified recombinant protein (HPV B19-VP1) coated in microtiter plate as immunosorbent .Results In this study ,we observed that the Cut-off value ,sensi-tivity ,and specificity of the IgM-ELISA established were 0 .25 ,84 .6% ,and 99 .7% respectively .The coincidence rate of determination results by the home made kit and control kit was 99 .47% .A total of 1700 serum samples from healthy donors and 115 serum samples from pregnant women were with HPV B19 IgM determined by the IgM-ELISA established ,in which the positive rates of B19 IgM were 1 .59% and 12 .17% respectively .Conclusion Our results suggested that the indirect ELISE-IgM ,in which HPV B19-VP1 were coated ,may be an appropriate assisted method to diagnose the early or acute phase of HPV B19 infection .关键词
人细小病毒B19/VP1结构蛋白/酶联免疫/辅助诊断Key words
human parvovirus B19/VP1 protein/ELISA/diagnostics引用本文复制引用
凌辉生,储迅涛,曾敏霞..人细小病毒B19IgM抗体间接ELISA检测方法的初步建立[J].检验医学与临床,2014,(5):583-585,588,4.基金项目
广东省教育部产学研结合项目（2011B090400535）。 （）
