昆明医科大学学报Issue(9)：7-12,6.
联合检测外周血CK19和SCCAg mRNA对早期宫颈癌患者微转移的诊断价值
The Diagnostic Value of Combined Detection of Peripheral Blood CK19 and SCCAg mRNA Expression in The Early Cervical Cancer Micrometastasis
摘要
Abstract
Objective Detect the change of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA in early cervical cancer patients before receiving radical hysterectomy by qRT-PCR technique, then study the value of combined detection of peripheral blood CK19 and SCCAg mRNA expression in diagnosis of early cervical cancer micrometastasis. Methods Thirty patients with early (ⅠA2 ~ⅡA) cervical cancer from the department of gynecological tumors in the third affiliated hospital of Kunming Medical University were selected as experimental group, 15 patients with uterine myoma and 15 healthy volunteers as negative control group, and 15 advanced (ⅡB~ⅢB) cervical cancer patients as positive control group. Peripheral blood samples before receiving radical resection of experimental group and the same samples before treatment of control groups were collected. The changes of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA were analyzed using qRT-PCR technique. Results There was significant difference in relative expression level of CK19 and SCCAg mRNA in the peripheral blood between experimental group and control groups ( <0.01) . And the positive rate of CK19 and SCCAg mRNA in the peripheral blood from 30 patients with early cervical cancer before operation in the experimental group was 30%(9/30) and 20% (6/30), respectively. Furthermore, combined detection of the expression of the two markers can improve the positive rate to 43.3%, much higher than single marker. There was also significant difference in the positive rate by combined detection of the two markers between the experimental group and control groups ( <0.05) . Conclusion CK19 and SCCAg mRNA could be used as the specific and suitable molecular markers for the detection of early cervical cancer micrometastasis. The combined detection of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA by qRT-PCR array could significantly enhance the sensitivity and specificity for cancer cell detection.关键词
宫颈癌/微转移/CK19/SCCAg/qRT-PCRKey words
Cervical cancer/Micrometastasis/CK19/SCCAg/qRT-PCR分类
医药卫生引用本文复制引用
魏向群,陈艳,李瑞敏,张磊..联合检测外周血CK19和SCCAg mRNA对早期宫颈癌患者微转移的诊断价值[J].昆明医科大学学报,2013,(9):7-12,6.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目（2010DC182) （）
