昆明医科大学学报Issue(10)：53-57,5.
白花蛇舌草提取物抗小鼠结直肠癌血管生成的实验研究
Inhibitory Effect of Ethanol Extract of Oldenlandia Diffusa on Colorectal Cancer Angiogenesis in BALB/c Mice
摘要
Abstract
Objective To explore the inhibitory effect of Oldenlandia diffusa extract on colorectal cancer angiogenesis in BALB/c mice. Methods Thirty-two BALB/c mice with subcutaneous CT26 colon cancer animal model were randomly equally divided into four groups,including the control group (groupⅠ,saline 0.1 mL/(10. d), O. diffusa ethanol extract of 90 mg/(kg.d) (groupⅡ), O. diffusa ethanol extract of 180 mg/(kg.d) (groupⅢ) and O. diffusa ethanol extract of 360 mg/(kg.d) (group Ⅳ) . Each group of mice were treated with intragastric administration of law administration 12 days after vaccination, then stopped and continue fed to 32 days, and the mice were killed. Micro-vascular dense ( MVD) was observed and countered under the microscopy by immunohistory chemistry. Results The murine colon tumor volumes of GroupⅡ,ⅢandⅣwere significantly less than that of groupⅠ,with significant difference ( <0.05) . The tumor microvessel density values of four groups was (7.83±2.87), (5.32±1.27), (1.77±0.70) and (1.87±0.68),respectively. The number of tumor blood vessels in GroupⅡ,Ⅲ and Ⅳ were significantly less than that of Ⅰ group, with significant difference ( <0.05) . Conclusion Within a certain dose range, the ethanol extract of O. diffusa can significantly inhibit the mouse colon cancer and the mechanism may be realated to inhibiting tumor angiogenesis.关键词
白花蛇舌草/BALB/c小鼠CT-26结直肠癌细胞株/BALB/c小鼠/结直肠癌/抑制血管生成Key words
Oldenlandia diffusa/BALB/c mice CT-26 colon cancer cell strain/BALB/c mice/Colorectal cancer/Inhibition of angiogenesis分类
医药卫生引用本文复制引用
肖云,伍治平,金从国,李佳,陈晓群,姚乾,朱启顺..白花蛇舌草提取物抗小鼠结直肠癌血管生成的实验研究[J].昆明医科大学学报,2013,(10):53-57,5.基金项目
云南省卫生厅科技计划项目 （）
