昆明医科大学学报Issue(10)：116-121,6.
早期宫颈癌患者术前外周血CK19 mRNA的检测及其临床意义
Detection of Early Cervical Cancer Patient’s Peripheral Blood CK19 mRNA before Surgery and its Clinical Significance
摘要
Abstract
Objective To investigate the detection of early cervical cancer patient's peripheral blood CK19 mRNA and its clinical significance. Methods 30 early (ⅠA2~ⅡA) cervical cancer patients from the department of gynecological tumors in the third affiliated hospital of Kunming Medical University were selected as experimental group, 15 patients with uterine myoma and 15 healthy volunteers as negative control group,15 advanced (ⅡB~ⅢB) cervical cancer patients as positive control group. Peripheral blood samples before receiving radical resection of experimental group,and the same samples before treatment of control groups were collected. The change of relative expression level and positive rate of peripheral blood CK19 was analyzed using qRT-PCR technique, and its correlation with clinicopathological parameters and prognosis was further analyzed. Results There was significant difference in relative expression level of CK19 mRNA in the peripheral blood between experimental group and control groups ( <0.001) . And the positive rate of CK19 in the peripheral blood from 30 patients with early cervical cancer before operation in the experimental group was 30% (9/30) . Furthermore, CK19 mRNA positive rate before surgery was correlated with lymph node metastasis, muscularis invasion and pathologic risk factors for postoperative ( <0.05) . There were no significant correlation between CK19 mRNA positive rate and other clinical and pathological features such as age,clinical stage (FIGO),tumor cell differentiation,tumor size,pathological type and etc ( > 0.05) . Conclusions CK-19 mRNA is a specific and suitable molecular marker for the detection of circulating tumor cells in early cervical cancer. Detection of peripheral blood CK19 mRNA expression before receiving radical hysterectomy in patients with early cervical cancer can also make up for the disadvantage of other methods such as the imaging, tumor marker detection. Most important of all, it can provide the basis for choosing a proper adjuvant therapy post-operation and estimating the relapse and prognosis.关键词
宫颈癌/循环肿瘤细胞/CK19/qRT-PCRKey words
Cervical cancer/Circulating tumor cells/CK19/qRT-PCR分类
医药卫生引用本文复制引用
魏向群,陈艳,李瑞敏,吴春秀,张芍..早期宫颈癌患者术前外周血CK19 mRNA的检测及其临床意义[J].昆明医科大学学报,2013,(10):116-121,6.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 （）
