昆明医科大学学报Issue(12)：1-4,25,5.
MMP-9表达在不同方法获得破骨细胞的比较研究
Comparison of MMP-9 Expression in Osteoclasts Obtained by Different Methods
摘要
Abstract
Objective To investigate the differences of protein and mRNA expression of matrix metalloproteinase 9 (MMP-9) in osteoclasts (OC) and osteoclast-like cells (OLC) obtained by mechanical and inducement methods. Methods Mechanical separation method was used to separate mature osteoclasts from long bones of SD rats aged one-day;and inducement culture method was applied to induce OLC by using RANKL (100 ng/mL) and M-CSF (100 ng/mL) . The protein expression of MMP-9 was measured by immunocytochemistry and mRNA expression of MMP-9 was assayed by in situ hybridization. Results The integral optical density (IOD) and average optical density (AOD) of positive cells in the visual field were higher in the 12-d group of inducement method as compared with the 3 d-group of mechanical method. Conclusions It is suggested that the protein and mRNA expression of MMP-9 in OLC obtained by 12 d inducement method is much high than in OC obtained by 3 d mechanical method. OLC obtained by inducement method can be applied in the study of osteoporosis.关键词
破骨细胞/破骨样细胞/机械法/诱导法/基质金属蛋白酶9Key words
Osteoclast/Osteoclast like cells/Mechanical method/Inducement method/Matrix metalloproteinase 9分类
医药卫生引用本文复制引用
杜明,何波,张小超,杨雪婷,陆义芹,陈鹏,沈志强..MMP-9表达在不同方法获得破骨细胞的比较研究[J].昆明医科大学学报,2013,(12):1-4,25,5.基金项目
国家自然科学基金资助项目（30660212、81160401、81260493）；云南省社会发展计划重点项目（2008CC009）；云南省应用基础研究基金资助项目 （）
