昆明医科大学学报Issue(1)：5-7,20,4.
蛋氨酸酶联合顺铂协同抑制人肺腺癌细胞GLC生长作用
Synergetic Inhibition Effects of Methioninase and Cisplatin against the Growth of Human Lung Adenocarcinoma Cell Line GLC
摘要
Abstract
Objective To purify recombinant methioninase and investigate the synergetic inhibitory effect of methioninase and cisplatin on the proliferation of human lung adenocarcinoma cell line GLC. Methods Recombinant methioninase was purified with GST-column from supernatant after ultrasonic disruption of cultured Escherichia coli in the prokaryotic expression system pGEX-4T-1-Met/Dh5a. MTT assay was used to determine the inhibition rate of methioninase in combined with cisplatin on cell proliferation,and their synergistic effect was evaluated by using the q value judge method. Results The concentration of recombinant methionine cleaving enzyme was 0.22 mg/mL, the purity was 95%, and the activity was 0.568 IU/mg. After 72 h culturing, the inhibition rate of cisplatin methionine was 24.80%and 27.49%respectively,while the inhibition rate of the combined drugs was 66.80% ( =1.47>1.15) which showed a significant synergistic effect. Conclusion Both methioninase and cisplatin have the inhibition effect,and the combined drugs display a significant synergistic effect on the proliferation of GLC.关键词
抑制/蛋氨酸裂解酶/协同/顺铂/肺腺癌细胞Key words
Inhibition/Methionine lyase/Synergy/Cisplatin/Lung adenocarcinoma cell分类
医药卫生引用本文复制引用
胡月新,徐天勇,张钰雯,赵瑜,田长富..蛋氨酸酶联合顺铂协同抑制人肺腺癌细胞GLC生长作用[J].昆明医科大学学报,2014,(1):5-7,20,4.基金项目
国家自然科学基金资助项目（30760287）；云南省科技厅自然科学基金资助项目 （）
