昆明医科大学学报Issue(1)：8-11,4.
醋酸棉酚对人舌鳞癌Tca8113细胞hMLH1蛋白及mRNA表达的影响
Effects of Gossypol Acetic Acid on Protein and mRNA Expressions of hMLH1 Gene in Human Tongue Carcinoma Cell Line Tca8113
摘要
Abstract
Objective The purpose of this study was to investigate the effects of gossypol acetic acid (GAA) on protein and mRNA expressions of hMLH1 gene in human tongue carcinoma cell line Tca8113 in vitro in order to discuss the mechanism of tumor suppression of GAA. Methods (1) Western-blot was used to study the effects of GAA on protein expressions of hMLH1 gene in Tca8113 cell line treated by different concentrations of GAA for 48 h. (2) Real-time fluorescence quantitative PCR (RFQ-PCR) was used to investigate the effects on the mRNA expressions of hMLH1 gene in Tca8113 cell line treated by GAA for 48 h. Results (1) Compared with the control group, the results of Western-blot showed that the protein expression of hMLH1 gene was increased after treatment by GAA for 48 h ( <0.05) . (2) The results of RFQ-PCR indicated that the mRNA expression of hMLH1 gene was increased after GAA treatment for 48 h ( <0.05) . Conclusion GAA could up-regulate protein and mRNA expression of hMLH1 in Tca8113 cell line, which indicated that it may be one of the mechanisms of tumor suppression effect of GAA.关键词
舌癌/Tca8113/醋酸棉酚/hMLH1Key words
Tongue neoplasms/Tca8113/Gossypol acetic acid/hMLH1分类
医药卫生引用本文复制引用
陈文飞,付帅,岳万远,吴勇..醋酸棉酚对人舌鳞癌Tca8113细胞hMLH1蛋白及mRNA表达的影响[J].昆明医科大学学报,2014,(1):8-11,4.基金项目
国家自然科学基金资助项目（81160326）；云南省科技计划项目 （）
