昆明医科大学学报Issue(1)：21-24,31,5.
SD大鼠髁突软骨下骨成骨细胞的原代培养与鉴定
Primary Culture and Identification of Osteoblasts from Subchondral Bone of Neonatal SD Rat's Condylar Process
摘要
Abstract
Objective Establish an experimental model for primary subchondral bone osteoblasts culture of neonatal SD rat's condylar process. Methods Under the condition of sterile,24-hour SD rat was executed and its condylar process was isolated. Removing cartilage layer, the subchondral bone was exposed obviously, then it was cultured with modified repeating enzymatic digestion-adherent explants method. The cellular morphology was identified with invert microscope and immunohistochemistry staining, the osteoblasts were identified by alkaline phosphorase (ALP) staining and calcified nodules staining, and the proliferation of the acquired cells was examined by methyl thiazolyl tetrazolium (MTT) assay. Results A variety of cell morphologies were observed, such as spindle-shaped, triangular and irregular-shape, and their cell processes were significant. The alkaline phosphatase staining and calcified nodules staining of cultured osteoblasts with mineralized nodules were positive. Cells grew slowly in 1-3 days, and the cells growth reached the highest level at the 8th day. The cells growth trend has gradually slowed down after 8 days. Conclusion The method is an efficient way to culture and obtain purified neonatal SD rat's subchondral bone osteoblasts with typical characteristics.关键词
软骨下骨/成骨细胞/细胞培养/鉴定Key words
Subchondral bone/Osteoblasts/Cell culture/Identification分类
医药卫生引用本文复制引用
曹小波,罗应伟,解保生,陈旭..SD大鼠髁突软骨下骨成骨细胞的原代培养与鉴定[J].昆明医科大学学报,2014,(1):21-24,31,5.基金项目
云南省应用基础研究面上基金资助项目 （）
