林产化学与工业Issue(1)：37-42,6.DOI:10.3969/j.issn.0253-2417.2014.01.007
纤维素前驱物6-13 C标记示踪研究纤维素与木质素连接方式
Analysis of the Association between Cellulose and Lignin by Carbon 13 Tracer Method with Cellulose Precursor
摘要
Abstract
In this paper, carbohydrate part of the lignin-carbohydrate complexes( LCC) in cell wall was labelled with 13C and traced with 13C NMR . Cellulose precursor, i. e. , uridine diphoshphate glucose-[6-13C], was synthesized, and injected into a living ginkgo tree together with lignin inhibitor AOPP and exogenous lignin precursor. It is proved that uridine diphoshphate glucose-[6-13C] is a suitable precursor for selective 13C-enrichment of cellulose in a plant. The specifically 13C-enriched LCC was isolated from the newly formed xylem of ginkgo shoots. Then it was degraded by cellulase and hemicellulase. Thus the lignin-rich fractions,which were called enzyme-degraded LCC ( EDLCC) , were obtained. By determining their FT-IR and 13C NMR spectra, information confirms that the bonds between C6 position of glucose units in cellulose andα-carbons of lignin side chain are benzyl ether linkage.关键词
纤维素/13C标记/13C NMR/木质素-碳水化合物复合体/苯甲醚键Key words
cellulose/13C isotopic tracer/13C NMR/lignin-carbohydrate complexes/benzyl ether linkage分类
化学化工引用本文复制引用
向松明,谢益民,杨海涛,姚兰..纤维素前驱物6-13 C标记示踪研究纤维素与木质素连接方式[J].林产化学与工业,2014,(1):37-42,6.基金项目
国家自然科学基金资助项目(31070521) （31070521）
